What is Alexa Fluor 488?
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.
Is Alexa Fluor 488 FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC.
What is the wavelength of Alexa Fluor 488?
Alexa Fluor® 488 is a green fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 496 nm, and an emission maximum around 519 nm.
Is Alexa Fluor a protein?
Our Alexa Fluor 488 protein A conjugate binds to the Fc portion of various immunoglobulins from several different species.
How long do Alexa Fluor antibodies last?
2 weeks
What temperature should I store the antibody at? Storage at 4°C should not exceed 1 or 2 weeks.
What is DyLight 488 and why is it essential?
DyLight® 488 is a green fluorophore commonly used in applications such as protein labeling, immunolabeling, fluorescence microscopy, and enzyme immunoassays. The dye has an absorption wavelength that peaks around 493 nm, and an emission maximum around 518 nm.
How long do fluorescent secondary antibodies last?
Storage at 4°C should not exceed 1 or 2 weeks.
How does Alexa Fluor work?
Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. The excitation and emission spectra of the Alexa Fluor series cover the visible spectrum and extend into the infrared.
What is FITC antibody?
FITC (fluorescein isothiocyanate) is a fluorochrome dye widely used as an antibody or other probe marker. FITC absorbs ultraviolet or blue light, exciting molecules which then emit a visible yellow-green light. When the excitation light is removed, the emission light ceases.
How long can you leave secondary antibody?
For incubation of the conjugated secondary antibody, a 1-hour incubation period at room temperature is usually enough and should not exceed 3 hours in order to avoid high background during the detection step.
What is FITC secondary antibody?
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target.
What does FITC stain for?
Fluorescein-5-isothiocyanate (FITC) is a fluorescence dye and belongs to the xanthene dyes. FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.
Why do you need a secondary antibody?
Using the same primary antibody in multiple different assays often requires a secondary antibody. The primary antibody detects the antigen in the specimen, but the secondary antibody can be designed to have a fluorophore or enzyme complex attached to it for the purposes of visualization.
Can you reuse secondary antibody?
Finally, very few researchers will reuse secondary antibodies, as you generally burn through fewer of them per equivalent volume. Secondary antibodies are also cheaper and used at far more dilute ranges. The decision to reuse antibodies overall is largely project dependent.
What is the peak of Alexa Fluor 488 antibody?
Alexa Fluor® 488 – conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488.
What can Alexa Fluor 488 dye conjugate to?
We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
What is the difference between Alexa Fluor 488 and Cy™2 and FITC?
In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media ( see Cy™2 for more information ).
How do anti-fluorescent dye antibodies work?
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence.